Pediococcus spp. qPCR Detection Kit (TBS42065)

$339.00$678.00

SKU: TBS42065 Categories: ,
SKUStock SIZE PriceQuantity
TBS42065-100 Yes 100 Reactions $339.00
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TBS42065-200 Yes 200 Reactions $678.00
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Description

Pediococcus spp. are gram-positive, catalase-negative, facultative anaerobic lactic acid bacteria. Pediococcus species are commonly found in food and beverage fermentation, and their detection is critical for quality control, contamination monitoring, and scientific research. The Tribioscience Pediococcus spp. qPCR Detection Kit is specifically designed to detect Pediococcus genus in a single real-time quantitative PCR (qPCR) reaction using fluorescent probes. Detection of the target DNA confirms ingredient authenticity and helps prevent food fraud to ensure food safety.

PRINCIPLE

The kit is based on probe real-time qPCR technology, which amplifies conserved gene sequences of Pediococcus species. The target-specific fluorescent probes bind to the amplified PCR products, and as the products accumulate, the fluorescence signal increases in PCR amplification. By monitoring fluorescence intensity in real time, the kit allows the quantitative detection of the target DNA during the PCR amplification and provides rapid and accurate results.

KEY FEATURES

• High sensitivity and specificity for all Pediococcus species.
• High efficiency: the optimal systemic conditions for PCR amplifications.
• Streamlined protocol: just add DNA Template and water.
• No cross reactivity with other species.

APPLICATIONS

qPCR detection of Pediococcus spp. is used in food and beverage production, fermentation process control, spoilage prevention, and microbial ecology studies to rapidly and specifically quantify the bacteria.

KIT CONTENTS

Name 100x rxn 200x rxn
qPCR Super Mix (B1) 0.9 mL 1.8 mL
Primer-probe Mix (B2) 0.5 mL 1.0 mL
Positive Control DNA (B+) 10 µL 20 µL
Negative Control DNA (B) 60 µL 120 µL

The Pediococcus spp. target probe has been labeled with FAM while the PCR internal control has been labeled with Hex.

The kit is shipped on ice and stored at -20°C for long-term storage. Shelf life of 12 months after receipt.

DNA Standard PCR amplification

DNA Standard Curve

Research use only.

Additional information

SIZE

200 Reactions, 100 Reactions

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