Pediococcus damnosus qPCR Detection Kit (TBS42066)

$339.00$678.00

SKU: TBS42066 Categories: ,
SKUStock SIZE PriceQuantity
TBS42066-100 Yes 100 Reactions $339.00
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TBS42066-200 Yes 200 Reactions $678.00
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Description

Pediococcus damnosus are gram-positive, catalase-negative, facultative anaerobic lactic acid bacteria. Pediococcus damnosus is commonly found in food and beverage fermentation, and their detection is critical for quality control, contamination monitoring, and scientific research.

The Tribioscience Pediococcus damnosus qPCR Detection Kit is specifically used to detect Pediococcus damnosus species in a single real-time quantitative PCR (qPCR) reaction using fluorescent probes. Detection of the target DNA confirms ingredient authenticity and helps prevent food fraud.

PRINCIPLE

The kit is based on probe real-time qPCR technology, which amplifies specific gene sequence of Pediococcus damnosus. Target-specific fluorescent probe is bonded to the amplified PCR products. As PCR products accumulate in PCR amplification, the fluorescence signal increases. By monitoring fluorescence intensity in real time, the kit allows the quantitative detection of the target DNA during the PCR amplification, providing rapid and accurate results.

KEY FEATURES

• High sensitivity and specificity for Pediococcus damnosus.
• High efficiency: the optimal systemic conditions for PCR amplifications.
• Streamlined protocol: just add DNA Template and water.
• No cross reactivity with other species.

APPLICATIONS

qPCR detection of Pediococcus damnosus is used in food and beverage production, fermentation process control, spoilage prevention, and microbial ecology studies to rapidly and specifically quantify the bacteria.

KIT CONTENTS

Name 100x rxn 200x rxn
qPCR Super Mix (B1) 0.9 mL 1.8 mL
Primer-probe Mix (B2) 0.5 mL 1.0 mL
Positive Control DNA (B+) 10 µL 20 µL
Negative Control DNA (B) 60 µL 120 µL

The Pediococcus damnosus target probe has been labeled with FAM while the PCR internal control has been labeled with Hex.

STORAGE CONDITION

The kit is shipped on ice and stored at -20°C for long-term storage. Shelf life of 12 months after receipt.

DNA Standard PCR amplification

DNA Standard Curve

Research use only.

Additional information

SIZE

200 Reactions, 100 Reactions

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