O157H7 E. coli qPCR Kit

Description

The O157H7 E. coli qPCR Kit has been designed to identify the O157H7 E. coli strain in a single PCR reaction using real-time quantitative polymerase chain reaction (qPCR) and probe fluorescence labels. The detection of the target DNA confirms ingredient authenticity and prevents food fraud, ethical issues, or health concerns.
The O157H7 E. coli qPCR Kit includes O157H7 target positive and negative controls, PCR internal controls labeled with Hex, a qPCR super mix, and the primer-probe mix in which the probe is labeled with Cy5 for O157H7 species. These aids in the straightforward interpretation of the results.

Applications:

Detect O157H7 target DNA in the plant, cannabis, cannabis ingredients, grain, food, herbals, and animal feed.

Key Features:

• High sensitivity and specificity for O157H7 E. coli detection.
• High efficiency: the optimal systemic conditions for PCR amplifications.
• Streamlined protocol: Just add DNA template and water.
• No cross reactivity with other species

 

Kit Contents:

Name	100x rxn	200x rxn
qPCR Super Mix	0.8mL	1.6mL
Primer-Probe Mix	0.6mL	1.2mL
Positive Control DNA	60μL	100μL
Negative Control DNA	60μL	100μL

The O157H7 probe has been labeled with Cy5 while the PCR internal control has been labeled with Hex.

Storage Conditions:

The kit is shipped on ice. All components should be stored at -20℃ in a non-frost-free freezer.
Shelf life is 12 months after receiving.

Additional information

SIZE	200 Reactions, 100 Reactions

Data Sheet:

TBS42026_O157H7_EColi_qPCR

MSDS:

TBS42026_015H7_ECOLI_QPCR_MSDS_2022