Clostridium butyricum qPCR Detection Kit (TBS42064)

$339.00$678.00

SKU: TBS42064 Categories: ,
SKUStock SIZE PriceQuantity
TBS42064-100 Yes 100 Reactions $339.00
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TBS42064-200 Yes 200 Reactions $678.00
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Description

Clostridium butyricum is a species of gram-positive, anaerobic, spore-forming bacterium. It is commonly found in soil, water, and the gastrointestinal tract of humans and animals. Its detection is critical for quality control, contamination monitoring, and scientific research. Tribioscience Clostridium cutyricum qPCR Detection Kit is specifically used to detect Clostridium butyricum species in a single real-time quantitative PCR (qPCR) reaction using fluorescent probes. Detection of the target DNA confirms ingredient authenticity and helps prevent food fraud to ensure food safety.

PRINCIPLE

The kit is based on probe real-time qPCR technology, which amplifies specific gene sequence of Clostridium butyricum. Target-specific fluorescent probes bind to the amplified PCR products. As the products accumulate, the fluorescence signal increases in PCR amplification. By monitoring fluorescence intensity in real time, the kit allows the quantitative detection of the target DNA during the PCR run and provides rapid and accurate results.

KEY FEATURES

• High sensitivity for Clostridium butyricum.
• High efficiency: the optimal systemic conditions for PCR amplifications.
• Streamlined protocol: just add DNA Template and water.
• No cross reactivity with other species.

APPLICATIONS

qPCR detection of Clostridium butyricum is used in food and environmental, fermentation process control, spoilage prevention, and microbial ecology studies to rapidly and specifically quantify the bacteria.

KIT CONTENTS

Name 100x rxn 200x rxn
qPCR Super Mix (B1) 0.9 mL 1.8 mL
Primer-probe Mix (B2) 0.5 mL 1.0 mL
Positive Control DNA (B+) 10 µL 20 µL
Negative Control DNA (B) 60 µL 120 µL

The Clostridium butyricum target probe has been labeled with FAM while the PCR internal control has been labeled with Hex.

STORAGE CONDITION

The kit is shipped on ice and stored at -20°C for long-term storage. Shelf life of 12 months after receipt.

DNA Standard Curve

DNA Standard Amplification

Research use only.

Additional information

SIZE

200 Reactions, 100 Reactions

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