CD39/ENTPD1 Activity Colorimetric Assay Kit (TBS2126)

$580.00

Target Synonym: ENTPD1, NTPDase-1, ATPDase, Ecto-ATPDase 1, Ecto-ATPase 1, Ecto-apyrase, ectonucleoside triphosphate diphosphohydrolase-1 (ENTPD1).

SKU: TBS2126 Category:
SKUStock SIZE (test) PriceQuantity
TBS2126-100 Yes 100 Assays $580.00
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Description

Cluster of Differentiation 39 (CD39/NTPDase-1) is a cell-surface ectonucleotidase prominently expressed on leukocytes and endothelial cells. By catalyzing the hydrolysis of extracellular ATP to ADP and AMP, CD39 serves as a critical regulator of purinergic signaling in cancer, inflammation, and immunological disorders. Consequently, its dysregulation is heavily implicated in neuroinflammation, cardiac dysfunction, obesity, and reproductive diseases.

 

Tribioscience’s CD39 Activity Kit provides a simple, easy assay that relies on quantification of free phosphate with Malachite Green complex. In this assay, CD39 in the presence of substrate generates free phosphate (Pi), which combines with malachite green reagents A (MG-A) and B (MG-B) to form the color complex shown in Fig. 1. The color intensity can be measured at 620 nm.

 

 

Applications

Measure CD39 activity in a variety of samples.

 

Kit Contents for 100 tests:

Part Name Part Size
CD39 Standard (400 µM) 0.2 mL
CD39 Assay Buffer 10 mL
CD39 Substrate 70 µL (10x)
CD39 Reagent A 1.3 ml
CD39 Reagent B 0.4 mL
CD39 Positive Control 12 µL (10x)

Store the Reagent at -20°C, protected from light, for 12 months.

 

Standard Curve

Research use only.

Additional information

SIZE (test)

100 Assays

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