Rat TNF-alpha ELISA Kit (TBS3054)
$460.00
Alternative names: TNF-α; Tumor Necrosis Factor α; TNF-alpha; TNF-a
| SKU | Stock | SIZE | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TBS3054 | Yes | 1 x 96-well plate | $460.00 |
- Description
- Terms
- Additional information
- Document
- Reviews (0)
Description
The Tribioscience Rat TNF-α ELISA is designed to quantitatively detect rat TNF-α levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 3 hours, not need 4-5 hours (Fig. 1). The detection range is from 62 to 4000 pg/mL. The levels of rat TNF-α samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural rat TNF-α protein.
Typical Data
This standard curve (R2=0.9998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MDD) of Rat TNF-α is typically 10 pg/ml.
The Intra-assay CV is < 10% the Inter-assay CV is < 12%.
Specificity
This assay recognizes natural and recombinant rat TNF-α.
No cross-reactivity with others.
Simple Procedures (Fig. 1)
Simple Standard Preparation (Fig.2)
Accurate Standard Curve (Fig.3 )
Kit Content and Storage Conditions
| PART | PART# | DESCRIPTION | STORAGE OF OPENED/ RECONSTITUTED |
| Rat TNF-α Microplate | TBS3054A | 96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for rat TNF-α. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Rat TNF-α Standard | TBS3054B | 30 µl of Recombinant rat TNF-α protein (200 ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3054C | 2.1 ml of Biotin-Rat TNF-α antibody. |
May be stored for up to 3 months at 2-8 °C. |
| Detection B | TBS3054D | 12 ml of Streptavidin-HRP. | |
| Assay Diluent | TBS3054E | 12 ml of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 ml of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 ml of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 ml of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
Research use only.
Additional information
| SIZE | 1 x 96-well plate, 2 x 96-well plate |
|---|
Datasheet:
TBS3054_Rat_TNF-alpha_2025_datasheet
MSDS:
TBS3054_Rat_TNF-alpha_ELISA_MSDS
Research use only.
Be the first to review “Rat TNF-alpha ELISA Kit (TBS3054)”
You must be logged in to post a review.
Reviews
There are no reviews yet.