(±)12(13)-DiHOME ELISA (TBS32129)
$460.00
| SKU | Stock | SIZE | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TBS32129 | Yes | 96-well plate | $460.00 |
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Description
(±)12(13)-DiHOME is the vicinal diol metabolite of (±)12(13)-EpOME (i.e., isoleukotoxin), an epoxide derived from linoleic acid via cytochrome P450 epoxygenase activity. It has been shown to suppress neutrophil respiratory burst activity and exhibit selective cytotoxicity in certain cell lines expressing sEH. Thus, (±)12(13)-DiHOME bridges lipid metabolism and immune modulation, acting both as a downstream stress-responsive signal and as a paracrine regulator in inflammation and energy homeostasis.
Intended Use
The Tribioscience’s (±)12(13)-DiHOME ELISA Kit utilizes competitive ELISA technologies for the quantitative analysis of (±)12(13)-DiHOME in biological samples. It provides a vital tool to accurately measure (±)12(13)-DiHOME with applications in inflammation, metabolism, and respiratory research. The assay has a range of 0.14-100 ng/ml.
Typical Data
This standard curve is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Standard Curve
Kit Content and Storage Conditions
| PART | PART# | DESCRIPTION | STORAGE OF OPENED/ RECONSTITUTED |
| Pre-coated Microplate | TBS32129A | 96 well microplate (12 strips of 8 wells) coated with an antibody specific for (±)12(13)-DiHOME. | Return unused wells to the foil pouch. Reseal along entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| (±)12(13)-DiHOME Standard | TBS32129B | 80 µl of (±)12(13)-DiHOME (1 µg/ml). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS32129C | 80 µl of HRP-(±)12(13)-DiHOME conjugate (100x). |
May be stored for up to 6 months at 2-8 °C. |
| Assay Diluent | TBS3000D | 25 ml of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 ml of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 ml of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 ml of 1 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration date.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
Research use only.
Additional information
| SIZE | 96-well plate |
|---|
Datasheet:
TBS32129_(±)12(13)-DiHOME ELISA_datasheet
MSDS:
TBS32129_(±)12(13)-DiHOME ELISA_MSDS
Research use only.
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