Creatinine amidohydrolase, Enzyme Activity (TBP0010)
$290.00 – $1,160.00
| SKU | Stock | SIZE | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TBP0010-1KU | Yes | 1000 U | $290.00 | |||
| TBP0010-5KU | Yes | 5000 U | $1,160.00 |
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Description
Preparation and Specification
Appearance: White amorphous powder, lyophilized
Activity: GradeⅡ (-211) 450U/mg-solid or more; GradeⅢ (-311) 150U/mg-solid or more
Contaminants: NADH oxidase ≤5.0×10⁻²%; Catalase ≤2.0%
Stabilizers: Sucrose, BSA
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: approx. 175,000
Isoelectric point: 4.7
Michaelis constants: 3.2×10⁻²M (Creatinine), 5.7×10⁻²M (Creatine)
Structure: 6 subunits per enzyme molecule (One zinc is bound to each subunit)
Inhibitors: Ag⁺, Hg⁺⁺, N-bromosuccinimide, EDTA
Optimum pH: 6.5-7.5
Optimum temperature: 70℃
pH Stability: pH 7.5-9.0 (5℃, 16hr)
Thermal stability: below 70℃ (pH 7.5, 30min)
Applications
This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase (CRH-211, CRH-221, CRH-229) and sarcosine oxidase (SAO-351) in clinical analysis.
Additional information
| SIZE | 1000 U, 5000 U |
|---|
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