Cyclic GMP Colorimetric Activity Assay (TBS2074)

$490.00

SKU: TBS2074 Category:
SKUStock SIZE (test) PriceQuantity
TBS2074-100 Yes 100 Tests $490.00
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Description

This assay kit is a competition enzyme-linked immunoassay designed to measure cGMP levels in cells or tissues. An anti-rabbit IgG was pre-coated onto a microplate. The cGMP in test sample competes with cGMP-HRP for binding to an anti-cGMP rabbit monoclonal antibody. Following the incubation and wash step, an ultra-sensitive substrate TMB is added for color development. Because of the competitive nature of this assay, the color intensity is inversely proportional to the quantity of sample cGMP levels. Measurement of absorbance using the cGMP Standard allows calculating the absolute amount of cGMP in a sample. This assay provides a flexible, accurate, simple, and time-saving approach for cGMP detection.

 

Typical Data

This standard curve is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 2 is an example of typical Data.

Sensitivity

The minimum detectable dose (MOD) of cGMP is typically 1 pM.

The Intra-assay CV and the Inter-assay CV are ˂10%.

Specificity

This assay recognizes cGMP. No cross-reactivity with others.

 

Standard Preparation

 

Standard Curve

Kit Content and Storage Conditions

Part Part# Description Storage of Opened/Reconstituted
Precoated Microplate TBS2074A 96 well polystyrene microplate (12 strips of 8 wells) coated with anti-mouse IgG antibody. Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
cGMP Standard TBS2074B 50 µL of cGMP (1 µM). Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A TBS2074C 110 µL of cGMP-HRP (25x). May be stored for up to 3 months at 2-8 °C.
Detection B TBS2074D 1.1 mL of anti-cGMP antibody (5x).
Assay Diluent TBS2074E 20 mL of a buffered protein base with preservatives.
Wash Buffer TBS3000W 12 mL of concentrated solution (10x).
TMB Substrate TBS3000T 12 mL of ultra-sensitive TMB substrate.
Stop Solution TBS3000S 6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.

Additional information

SIZE (test)

100 Tests

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