Lentivirus qPCR Titer Kit (TBS4052)

$295.00

TribioScience’s Lentivirus (LV) qPCR Titer Kit incorporates TribioScience FAST technology paradigm and probe Taqman qPCR technology for an accurate, one-step, and time-saving qPCR titer determination of lentivirus.

SKU: TBS4052 Category:
SKUStock SIZE (test) PriceQuantity
TBS4052-100 Yes 100 Tests $295.00
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Description

TribioScience’s Lentivirus (LV) qPCR Titer Kit incorporates TribioScience FAST technology paradigm and probe Taqman qPCR technology for an accurate, one-step, and time-saving qPCR titer determination of lentivirus. The probe is labelled with Fluorescence FAM. The entire procedure, which does not require additional reverse transcription step, can be completed within 2 hours. With its time-saving 1-minute enzyme activation technology, specificity, and sensitivity, the performance of TribioScience’s Lentivirus qPCR Titer Kit is unmatched by similar kits currently on the market.

 

Kit Content for 100RXN

Component Volume Part Number
FAST Lysis Buffer 1.0 mL LV001
Fast qPCR mix 1.0 mL LV002
Primer-Probe Mix 0.3 mL LV003
LV Control DNA 30 µL LV004
PCR Buffer 1.0 mL LV005

Note: The probe is labelled with Fam.

 

Storage and Shelf-life

Store at -25⁰C to -15⁰C immediately upon arrival. Minimize the number of freeze-thaw cycles to ensure superior performance. The Kit is stable for one (1) year from the date of arrival.

Table 1: Standard Preparation
Dilution Virus Titer (IU/ml)
1/10 1×107
1/100 1×106
1/1000 1×105
1/10000 1×104
1/100000 1×103

Table 2: qPCR Reaction Setup
Component Volume (µL)
Fast qPCR mix 10.0
Primer-Probe mix 2.0
DNA Sample or Standard, NTC 2.0
PCR Buffer 6.0
Final Volume 20

Table 3: PCR Conditions
Purpose Temperature (℃) Time Cycle(s)
Enzyme Activation 94 60S 1
Denaturation 94 10S 40
Priming/Extension 60 60S

Research use only.

Additional information

SIZE (test)

100 Tests

1 review for Lentivirus qPCR Titer Kit (TBS4052)

  1. 5 out of 5

    jianxinh

    the result is very precise. the protocol is user-friendly. manual is clear


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