blaOXA-58 qPCR Kit (TBS42062)

Description

blaOXA-58 is a gene that can hydrolyze β-lactam antibiotics, including penicillins, cephalosporins, and carbapenems, rendering these antibiotics ineffective against bacteria harboring this gene. The gene is often located on plasmids, facilitating its transfer between bacteria and contributing to the spread of antibiotic resistance. Therefore, research on blaOXA-58 is vital for guiding the development of treatments targeting resistant bacteria.

Principle

Authenticating ingredients utilizes real-time PCR which is based on the amplification of a specific region of the relevant target genome. The amplified product is detected using target-specific fluorescent probes that bind to the amplified product. As the PCR product accumulates, there is an increased fluorescent signal from the bound probes. Monitoring the fluorescence intensities during the PCR run allows the detection of the accumulating PCR product in real time.

Tribioscience’s blaOXA-58 qPCR Kit includes blaOXA-58 positive and negative controls, PCR internal controls labeled with Hex, a qPCR super mix, and the primer-probe mix in which the probe has been labeled with FAM for the target gene. These aid in a straightforward interpretation of the results.

Key Features

❖ High sensitivity and specificity for blaOXA-58 gene.
❖ High efficiency: the optimal systemic conditions for PCR amplifications.
❖ Streamlined protocol: just add DNA Template and water.
❖ No cross reactivity with other genes.

Applications

Detect blaOXA-58 gene in clinical specimens, environmental samples, and bacterial isolates.

Kit Contents

The blaOXA-58 target probe has been labeled with FAM while the PCR internal control has been labeled with Hex.

Storage Conditions

The kit is shipped on ice and stored at -20℃ for long-term storage.
Shelf life of 12 months after receiving.

Research use only.

Additional information

Data Sheet:

TBS42062_blaOXA-58_qPCR kit_2025

MSDS:

TBS42062_blaOXA-58_QPCR_MSDS_2025

Research use only.