Avian Influenza A Virus (AIV) H5-H7-H9 One-Step RT-qPCR (TBS42039)

$590.00

SKU: TBS42039 Category:
SKUStock SIZE (test) PriceQuantity
TBS42039-100 Yes 100 Tests $590.00
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Description

Avian Influenza Virus (AIV) TaqMan Probe RT-PCR Detection Kit is one step real-time reverse transcription polymerase chain reaction (RT-PCR) assay intended for the simultaneous detection of AIV H5, H7 and H9 subtypes in avian samples in one reaction tube. It combines both reverse transcription (RT) and Taqman probe-based PCR amplification to occur in a single reaction tube. The kit is optimized for the two reactions in a real-time “single step”. This One-step qRT-PCR assay offers the end-users an efficient, easy to use, and reliable alternative to conventional “two-step” sequential qRT-PCR for detecting target.

 

AIV TaqMan Probe RT-PCR Detection Kit contains all components for RT-PCR reaction including RT-PCR enzymes, primer-probes, internal control, positive control, negative control, and buffer. The AIV target genes H5, H7, and H9 are labeled with fluorescence Fam, and internal control is labeled with Hex.

 

Applications

This kit is used for the detection of AIV H5, H7, and H9 subtypes in avian samples.

 

Key Features

  • One-step complete qRT-PCR in a single tube.
  • Reduce contamination in the operating process.
  • Accurate and simultaneous detection of AIV H5, H7, and H9 subtypes.
  • 4x RT-PCR mix makes it easier to adjust the sample size in a one-tube reaction.

 

Kit contents

Component 100RXN
5X RT-PCR Master Mix 0.5 mL
10X AIV Primer-probe Mix 0.21 mL
AIV Positive Control 30 µL
AIV Negative Control 30 µL
RT-PCR Buffer 0.7 mL
DEPC Water 1.0 mL

 

STORAGE CONDITIONS

Store all components at -20°C in a non-frost-free freezer.

Shelf life is 12 months after recipient.

The kit is shipped on ice.

Research use only.

Additional information

SIZE (test)

100 Tests

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