Xanthine oxidase, Enzyme Activity (TBP0045)

Description

Preparation and Specification

Appearance: Reddish brown amorphous powder, lyophilized

Activity: GradeⅡ 10U/mg-solid or more

Contaminants: Catalase ≤5%

Adenosine deaminase ≤1.0×10⁻³%

Uricase ≤1.0×10⁻³%

Phosphatase ≤1.0×10⁻³%

Purine-nucleoside phosphorylase ≤5.0×10⁻³%

Stabilizers: Sodium glutamate, BSA

 

Properties

Stability: Stable at -20°C for at least One year

Molecular weight: approx. 160,000

Isoelectric point: 4.0±0.1

Michaelis constants: 4.5×10⁻⁵M (Xanthine), 7.6×10⁻⁵M (Hypoxanthine)

Inhibitors: Reducing agents, Hg⁺⁺, Ag⁺,MIA

Optimum pH: 7.5-8.0

Optimum temperature: 65°C

pH Stability: pH 6.5-9.0 (25°C, 15hr)

Thermal stability: below 55°C (pH 8.0, 30min)

 

Applications

This enzyme is useful for enzymatic determination of inorganic phosphorus, 5′-nucleotidase and adenosine deaminase when coupled with Purine-nucleoside phosphorylase (PNP-311) and uricase (UAO-201, UAO-211).

Additional information

SIZE	50 U, 100 U

Datasheet:

TBP0045-Xanthine oxidase

MSDS:

TBP0045_Xanthine oxidase Safety Data Sheet