Creatine amidinohydrolase, Enzyme Activity (TBP0014)
$252.00 – $1,009.00
SKU | Stock | SIZE | Price | Quantity | ||
---|---|---|---|---|---|---|
TBP0014-1KU | Yes | 1000 U | $252.00 | |||
TBP0014-5KU | Yes | 5000 U | $1,009.00 |
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Description
Preparation and Specification
Appearance: (-221) White amorphous powder, lyophilized (-229) Clear solution
Activity: GradeⅡ (-221) 4.0U/mg-solid or more (-229) 2000U/ml or more
Contaminants: NADH oxidase ≤5.0×10⁻²%; Catalase ≤2.0%
Stabilizers: Sugars, EDTA
Properties
Stability: (-221) Stable at -20°C for at least One year (-229) Stable at 4°C
Molecular weight: approx. 67,000 (by gel filtration)
Structure: 2 subunits per enzyme molecule
Isoelectric point: 4.5±0.1
Michaelis constant: 4.5×10⁻³ M (Creatine)
Inhibitors: Hg⁺⁺, Cu⁺⁺, Ag⁺, SH reagent (NEM), PCMB
Optimum pH: 6.5-7.5
Optimum temperature: 40-50℃
pH Stability: pH 4.0-10.0 (25℃, 20hr)
Thermal stability: below 50℃ (pH 7.5, 30min)
Applications
This enzyme is useful for enzymatic determination of creatine and creatinine when coupled with creatinine amidohydrolase (CNH-211, CNH-311) and sarcosine oxidase (SAO-351) in clinical analysis.
Additional information
SIZE | 1000 U, 5000 U |
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