a-Glucosidase, Enzyme Activity (TBP0049)
$234.00 – $936.00
| SKU | Stock | SIZE | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TBP0049-1KU | Yes | 1000 U | $234.00 | |||
| TBP0049-5KU | Yes | 5000 U | $936.00 |
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Description
Preparation and Specification
Appearance: White amorphous powder, lyophilized
Activity: GradeⅡ 20U/mg-solid or more
Contaminants: α-amylase ≤1.0×10-5%
Stabilizers: BSA
Properties
Stability: Stable at −20℃ for at least one year
Molecular weight: approx. 65,000 (Gel-filtration and SDS-PAGE)
Isoelectric point: 5.2
Michaelis constant: 6.3×10-4M (p-Nitrophenyl-α-D-glucopyranoside)
Inhibitors: Ag＋, Hg＋＋, PCMB, MIA
Optimum pH: 6.0−7.0
Optimum temperature: 60℃
pH Stability: pH 5.0−9.0
Thermal stability: below 60℃ (pH 7.0, 15min)
| Substrate* | Relative hydrolysis rate** | Substrate* | Relative hydrolysis rate** |
| PNPG | 100.0 | Maltose | 271.0 |
| PNPG2 | 205.0 | Maltotriose | 203.0 |
| PNPG3 | 284.0 | Maltotetraose | 168.0 |
| PNPG5 | 164.0 | Maltopentaose | 100.0 |
* : Substrate concn. 2.2mM
** : Glucose-forming activity, pH 6.8 at 37℃
Effect of various chemicals: (Table 1)
Applications
This enzyme is useful for structural investigations of carbohydrates and for the enzymatic determination of α-amylase when coupled with hexokinase (HXK-311) and G-6-P dehydrogenase (G6D-311, G6D-321) in clinical analysis.
Additional information
| SIZE | 1000 U, 5000 U |
|---|
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