Colipase, Enzyme Activity (TBP0063)
$549.00 – $2,196.00
SKU | Stock | SIZE (mg) | Price | Quantity | ||
---|---|---|---|---|---|---|
TBP0063-1MG | Yes | 1 MG | $549.00 | |||
TBP0063-5MG | Yes | 5 MG | $2,196.00 |
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Description
Product Details
Form: Freeze-dried powder
Solubility: Water or dilute buffer
Stability: Store at -20° C (-4° F)
Activity: 4000-12000 U/mg solid
Protein: 95%
Contaminants: Free of lipase
Catalog No.: 142A4000
Unit Definition
The amount of enzyme causing the release of one micromole of fatty acid from tributyrin per minute at 25°C.
Applications
Pancreatic lipase when prepared essentially free of co-lipase is strongly inhibited by conjugated bile salts at or above their critical micell concentration. Addition of co-lipase restores activity to the bile-salt-inhibited lipase and gelfilteration experiments indicate that co-lipase in bile salt solution makes a dimer which forms a 1:1 complex with lipase. Lipase and co-lipase interact as a stoichiometrical relationship and classified co-lipase interact as a co-enzyme for lipase. The co-lipase is a single chain polypeptide with a molecular weight approximately 11,000 with 5 disulfide bridges and isoelectric point 5.0.
Reagents
- Tributyrin (reagent grade).
- NaOH, 0.2 M.
- Lipase (50 Units).
- Reaction mixture. Dissolve 0.24 g Tris, 2.09 g Sodium tauro-deoxycholate, 0.11 g CaCl2 and 8.76 g NaCl in 800 ml of distilled H2O. Adjust pH to 6.5 with HCl. Bring volume to 1000 ml with distilled H2O.
Procedure
- Into the reaction vessel of an automatic titrator pipette 15 ml of the reaction mixture and 0.5 ml of Tributyrin at 25°C.
- Add 50 potentiometric units of lipase to the reaction vessel.
- Maintaining the pH at 6.5 (and temperature at 22-24°C), titrate the mixture with 0.2 M NaOH over a 5 min period.
- Calculate the blank rate.
- To the reaction vessel add 75 μl of a colipase sample that has been diluted 1:10 with distilled H2O.
- Titrate the reaction for 5 minutes.
- Calculate the rate of reaction. (if the net rate is 0.040-0.095 ml/min., continue with the calculation. If the rate is outside this range, adjust the concentration of colipase and reanalyze).
Additional information
SIZE (mg) | 1 MG, 5 MG |
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