Ascorbate oxidase, Enzyme Activity (TBP0006)
$186.00 – $744.00
SKU | Stock | SIZE | Price | Quantity | ||
---|---|---|---|---|---|---|
TBP0006-1KU | Yes | 1000 U | $186.00 | |||
TBP0006-5KU | Yes | 5000 U | $744.00 |
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Description
Preparation and Specification
Appearance: Light blue amorphous powder, lyophilized
Activity: GradeⅢ 200U/mg-solid or more (containing approx. 70% of stabilizers)
Contaminants: Catalase ≤1.0×10-1%; Phosphatase ≤2.0×10-2%
Stabilizers: BSA, borax, basic amino acids.
Properties
Stability: Stable at −20℃ for at least one year
Molecular weight: 132,000, 140,000
Isoelectric point: between 6.0 and 7.8, 8.2
Michaelis constant: 2.5×10-4M (Ascorbate)
Structure: 8 copper atoms per enzyme molecule
Inhibitors: cyanide, Na2S, diethyldithiocarbamate (Na)
Optimum pH: 5.6
Optimum temperature: approx. 30℃
pH Stability: pH 7.0−10.0 (25℃, 17hr)
Thermal stability: below 40℃ (pH 8.0, 30min)
Substrate specificity: This enzyme oxidizes ascorbic acid and several ascorbic derivatives.
Applications
This enzyme is useful for enzymatic determination of ascorbic acid and for eliminating the interference of ascorbic acid in clinical analysis.
Additional information
SIZE | 1000 U, 5000 U |
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