Adenosine Deaminase, Enzyme Activity (TBP0052)

Description

Product Details

Form: Freeze-dried powder

Solubility: Distilled water or dilute buffer

Stability: Store at -20° C (-4° F)

Activity: 3 U/mg protein

Protein: 95%

Catalog No.: 212A0200

 

Unit Definition

One unit will deaminate 1.0 &mgr;mole of adenosine to inosine per min at pH 7.4 at 25° C.

 

Reagents

1. 0.05 M Phosphate buffer, pH 7.4.
2. Adenosine, 3.6 mg/ml dissolved in buffer.
3. Enzyme solution in buffer (1-2 U/ml).

 

Procedure

1. Set up spectrophotometer (with strip chart and temp. control) to 265 nm and 25° C.
2. Into a cuvette, pipette the following:

Phosphate buffer 2.97 ml

Adenosine            0.01 ml

Incubate cuvette in spectrophotometer at 25° C for 5 minutes to attain temperature equillibration and then establish blank rate if any.

3. Initiate reaction by adding 0.02 ml enzyme solution.
4. Calculate E265nm/min

 

Applications

Adenosine Deaminase (EC 3.5.4.4) is widely distributed in animal tissues. The highest enzyme activities are found in the mucosa of the small intestine, in the appendix and the spleen. The enzyme is present in the cytoplasmic cell fraction and also to some extent in the nucleus. The optimum pH is 7.0 to 7.4.

 

 

Additional information

SIZE	1000 U, 250 U

Datasheet:

TBP0052-Adenosine Deaminase

MSDS:

TBP0052_Adenosine Deaminase Safety Data Sheet