Human IL-17A ELISA; 1 step and 1hr

$390.00

This kit utilizes a simple ELISA format to detect IL-17A in cell culture supernatant, plasma, and serum in less than a couple hours.

SKU: TBS3228 Category:
SKUStock SIZE PriceQuantity
TBS3228 Yes 1 x 96-well plate $390.00
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Description

The Fast Human IL-17A ELISA is a solid phase ELISA designed to measure human IL-17A levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not 5-6 hours (Fig. 1). The detection range is from 8 to 2000pg/mL. The levels of human IL-17A samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-17A protein.

 

Typical Data

This standard curve(R2=0.998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Sensitivity

The minimum detectable dose (MOD) of human IL-17A is typically 6.5 pg/ml. The Intra-CV is 5.39%, and the Inter-CV is < 12%.

Specificity

This assay recognizes natural and recombinant human IL-17A.
No-cross reactivity: Human INF-γ, IL-10, IL-12, IL-16, IL-17B, IL-17C, IL-17D, IL-17F; Mouse IL-17.

 

Simple Procedures (Fig. 1)

 

Simple Standard Preparation

 

Accurate Standard Curve

 

Kit Content and Storage Conditions

Part Part# Description Storage of Opened/Reconstituted
Human IL-17A
Microplate
TBS3228A 96-well microplate (12 strips of 8 wells) coated with a
Capture Antibody specific for human IL-17A.
The unused wells can be stored in the sealed foil pouch
containing the desiccant pack for up to 1 month at 2-8 °C.
Human IL-17A
Standard
TBS3228B 60 μL of Recombinant human L-17A protein (100ng/mL). Aliquot and store at -20 ℃ for up to 1 month in a manual defrost
freezer. Avoid repeated freeze-thaw cycles.
Detection A TBS3228C 2.2 mL of human IL-17A antibody. May be stored for up to 3 months at 2-8 °C.
Detection B TBS3228D 120 μL of Streptavidin-HRP.
Assay Diluent TBS3228E 12 mL of a buffered protein base with preservatives.
10x Wash Buffer TBS3000W 12 mL of concentrated solution (10x).
TMB Substrate TBS3000T 12 mL of ultra-sensitive TMB substrate.
Stop Solution TBS3000S 6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use an expired kit for assay.
The kit contains sufficient materials to run an ELISA on one 96-well plate.

 

Related Product

  1. Human IL-1ß ELISA (TBS3219)
  2. Human IL-2 ELISA (TBS3220)
  3. Human IL-4 ELISA (TBS3221)
  4. Human IL-6 ELISA (TBS3223)
  5. Human IL-7 ELISA (TBS3224)

Additional information

SIZE

1 x 96-well plate

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