Fast Human IL-12 p70 ELISA
The Fast Human IL-12 p70 ELISA is a solid phase ELISA designed to measure human IL-12 p70 levels in cell culture supernatants, serum, and plasma.
|TBS3204||Yes||1 X 96-Well Plate||$420.00|
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Interleukin 12 (IL-12) is an anti-inflammatory cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit, also known as a natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF). The IL-12 and IL-23 (another heterodimeric cytokine) share the p40 subunit. IL-12 inhibits the synthesis of numbers of cytokines, including IFN-gamma, IL-2, IL-3, TNF, and GM-CSF produced by activated macrophages and by helper T-cells.
This standard curve(R2=0.998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
The minimum detectable dose (MOD) of human IL-12 p70 is typically 6.5 pg/ml.
The Intra-CV is < 10%, the Inter-CV is < 12%.
This assay recognizes natural and recombinant human IL-12 P70.
Human IL-12 p35; Human IL-12/IL-23 p40; Human IL-23; Mouse IL-12 p70.
SIMPLE STANDARD PREPARATION
KIT CONTENT AND STORAGE CONDITIONS
|PART||PART#||DESCRIPTION||STORAGE OF OPENED/ RECONSTITUTED|
|Human IL-12 Microplate||TBS3204A||96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human IL-12 p70.||The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.|
|Human IL-12 Standard||TBS3204B||30 μL of Recombinant human IL-12 protein (100ng/mL).||Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.|
|Detection A||TBS3204C||2.1 mL of human IL-12 antibody.|| |
May be stored for up to
3 months at 2-8 °C.
|Detection B||TBS3204D||12 mL of Streptavidin-HRP|
|Assay Diluent||TBS3204E||12 mL of a buffered protein base with preservatives.|
|10x Wash Buffer||TBS3000W||12 mL of concentrated solution (10x).|
|TMB Substrate||TBS3000T||12 mL of ultra-sensitive TMB substrate.|
|Stop Solution||TBS3000S||6 mL of 2 N sulfuric acid.|
1 X 96-Well Plate