Fast Human IL-12 p70 ELISA

Description

The Fast Human IL-12 p70 ELISA is a solid phase ELISA designed to measure human IL-12 p70 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, with no need for 5-6 hours (Fig. 1). The detection range is from 8 to 2000 pg/mL. The levels of human IL-12 p70 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-12 protein.

 

TYPICAL DATA

This standard curve (R²=0.998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

SENSITIVITY

The minimum detectable dose (MOD) of human IL-12 p70 is typically 6.5 pg/ml.

The Intra-CV is < 10% and the Inter-CV is < 12%.

SPECIFICITY

This assay recognizes natural and recombinant human IL-12 P70.

No-cross reactivity: Human IL-12 p35; Human IL-12/IL-23 p40; Human IL-23; Mouse IL-12 p70.

 

SIMPLE PROCEDURES (Fig. 1)

SIMPLE STANDARD PREPARATION

 

 

STANDARD CURVE

 

KIT CONTENT AND STORAGE CONDITIONS

PART	PART#	DESCRIPTION	STORAGE OF OPENED/ RECONSTITUTED
Human IL-12 Microplate	TBS3204A	96-well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human IL-12 p70.	The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.
Human IL-12 Standard	TBS3204B	Recombinant human IL-12 protein (Lyophilized). Reconstitute to 0.5 mL Assay Buffer.	Store at 2-8 °C for 6 months. After reconstituting in 0.5mL Assay Buffer, it can be stored at -20 ℃ for up to 1 month.
Detection A	TBS3204C	2.1 mL of human IL-12 antibody.	May be stored for up to 6 months at 2-8 °C.
Detection B	TBS3204D	0.25 mL of Streptavidin-HRP (1:50 Dilution).
Assay Diluent	TBS3204E	25 mL of a buffered protein base with preservatives.
10x Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

 

RELATIVE PRODUCTS

1. Human IL-1ß ELISA (TBS3219)
2. Human IL-2 ELISA (TBS3220)
3. Human IL-4 ELISA (TBS3221)
4. Human IL-6 ELISA (TBS3223)
5. Human IL-7 ELISA (TBS3224)
6. Human IL-8 ELISA (TBS3225)

Additional information

SIZE (kit)	1 x 96-Well Plate

Datasheet:

TBS3204_human_IL12 p70_elisa_datasheet_V02

MSDS:

TBS3204_Human-IL12P70ELISA_MSDS