Fast Human IL-12 p70 ELISA

Fast Human IL-12 p70 ELISA

$420.00

The Fast Human IL-12 p70 ELISA is a solid phase ELISA designed to measure human IL-12 p70 levels in cell culture supernatants, serum, and plasma.

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SKUStock SIZE (kit) PriceQuantity
TBS3204Yes1 X 96-Well Plate $420.00
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Description

Interleukin 12 (IL-12) is an anti-inflammatory cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit, also known as a natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF). The IL-12 and IL-23 (another heterodimeric cytokine) share the p40 subunit. IL-12 inhibits the synthesis of numbers of cytokines, including IFN-gamma, IL-2, IL-3, TNF, and GM-CSF produced by activated macrophages and by helper T-cells.

 

TYPICAL DATA

This standard curve(R2=0.998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

SENSITIVITY

The minimum detectable dose (MOD) of human IL-12 p70 is typically 6.5 pg/ml.

The Intra-CV is < 10%, the Inter-CV is < 12%.

SPECIFICITY

This assay recognizes natural and recombinant human IL-12 P70.

No-cross reactivity:

Human IL-12 p35; Human IL-12/IL-23 p40; Human IL-23; Mouse IL-12 p70.

 

SIMPLE PROCEDURES

SIMPLE STANDARD PREPARATION

 

STANDARD CURVE

 

KIT CONTENT AND STORAGE CONDITIONS

PARTPART#DESCRIPTIONSTORAGE OF OPENED/ RECONSTITUTED
Human IL-12 MicroplateTBS3204A96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human IL-12 p70.The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.
Human IL-12 StandardTBS3204B30 μL of Recombinant human IL-12 protein (100ng/mL).Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection ATBS3204C2.1 mL of human IL-12 antibody. 

May be stored for up to

3 months at 2-8 °C.

Detection BTBS3204D12 mL of Streptavidin-HRP
Assay DiluentTBS3204E12 mL of a buffered protein base with preservatives.
10x Wash BufferTBS3000W12 mL of concentrated solution (10x).
TMB SubstrateTBS3000T12 mL of ultra-sensitive TMB substrate.
Stop SolutionTBS3000S6 mL of 2 N sulfuric acid.

 

RELATIVE PRODUCTS

  1. Human IL-1ß ELISA (TBS3219)
  2. Human IL-2 ELISA (TBS3220)
  3. Human IL-4 ELISA (TBS3221)
  4. Human IL-6 ELISA (TBS3223)
  5. Human IL-7 ELISA (TBS3224)
  6. Human IL-8 ELISA (TBS3225)

Additional information

SIZE (kit)

1 X 96-Well Plate

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