Fast Human IL-12 p70 ELISA
$420.00
The Fast Human IL-12 p70 ELISA is a solid phase ELISA designed to measure human IL-12 p70 levels in cell culture supernatants, serum, and plasma.
| SKU | Stock | SIZE (kit) | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TBS3204 | Yes | 1 X 96-Well Plate | $420.00 |
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Description
Interleukin 12 (IL-12) is an anti-inflammatory cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit, also known as a natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF). The IL-12 and IL-23 (another heterodimeric cytokine) share the p40 subunit. IL-12 inhibits the synthesis of numbers of cytokines, including IFN-gamma, IL-2, IL-3, TNF, and GM-CSF produced by activated macrophages and by helper T-cells.
TYPICAL DATA
This standard curve(R2=0.998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
SENSITIVITY
The minimum detectable dose (MOD) of human IL-12 p70 is typically 6.5 pg/ml.
The Intra-CV is < 10%, the Inter-CV is < 12%.
SPECIFICITY
This assay recognizes natural and recombinant human IL-12 P70.
No-cross reactivity:
Human IL-12 p35; Human IL-12/IL-23 p40; Human IL-23; Mouse IL-12 p70.
SIMPLE PROCEDURES
SIMPLE STANDARD PREPARATION
STANDARD CURVE
KIT CONTENT AND STORAGE CONDITIONS
| PART | PART# | DESCRIPTION | STORAGE OF OPENED/ RECONSTITUTED |
| Human IL-12 Microplate | TBS3204A | 96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human IL-12 p70. | The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C. |
| Human IL-12 Standard | TBS3204B | 30 μL of Recombinant human IL-12 protein (100ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3204C | 2.1 mL of human IL-12 antibody. | May be stored for up to 3 months at 2-8 °C. |
| Detection B | TBS3204D | 12 mL of Streptavidin-HRP | |
| Assay Diluent | TBS3204E | 12 mL of a buffered protein base with preservatives. | |
| 10x Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
RELATIVE PRODUCTS
Additional information
| SIZE (kit) | 1 X 96-Well Plate |
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