Human Glucagon ELISA

Human Glucagon ELISA


Human Plasma/Serum Glucagon

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SKUStock SIZE PriceQuantity
TBS3244-01Yes1x96-well plate $490.00


Glucagon is a 29 amino acid polypeptide hormone, produced in the pancreas. It plays a vital role in glycogenolysis, lipolysis, gluconeogenesis, and ketogenesis. The Glucagon precursor mRNA is expressed by alpha cells of the pancreas, L cells of the intestine, and in the brain, but only the pancreatic alpha -cells are able to produce Glucagon. In normal metabolism, Glucagon normally affects on responding to low blood glucose (hypoglycemia) and is downregulated in response to high blood glucose (hyperglycemia).

Tribioscience’s Fast Human Glucagon ELISA is a solid phase ELISA designed to measure glucagon levels in cell culture supernatants. serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to make easy combinations of samples and detection into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and ultra-sensitive. The measurement can detect the concentration as low as 10 pg/mL, and finished in 2 hours, not need 5-6 hours (Fig. 1). The detection arrangement is from 10 to 2000 pg/mL. The levels of human glucagon samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human glucagon.


Kit Content and Storage Conditions

PartPart#DescriptionStorage of Opened/Reconstituted
Human Glucagon MicroplateTBS3244A96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human glucagon.Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human Glucagon StandardTBS3244B50 µl of Recombinant human glucagon (50ng/mL).Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.
Detection ATBS3244C2.1 ml of HRP- human glucagon antibody.May be stored for up to 3 months at 2-8 °C.
Assay DiluentTBS3244D12 ml of a buffered protein base with preservatives.
Wash BufferTBS3000W12 mL of concentrated solution (10x).
TMB SubstrateTBS3000T12 mL of ultra-sensitive TMB substrate.
Stop SolutionTBS3000S6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.


Standard Curve

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Additional information


1×96-well plate


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