Fast Human TGF-β1 ELISA
$450.00
SKU | Stock | SIZE (kit) | Price | Quantity | ||
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TBS3232 | Yes | 1 Kit | $450.00 |
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Description
The Fast Human TGF-β1 ELISA is a solid phase ELISA designed to measure human TGF-β1 levels in cell culture supernatants, serum, and plasma. The main feature of this kit is its use of a novel fast approach to replace traditional methods. The kit is designed to ensure short steps, a short incubation time, and only one set washing out. The measurement can be finished within 2 hours instead of the traditional 4-5 hours. The detection range is between 10-4,000 pg/mL. The levels of human TGF-β1 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human TGF-β1 protein.
Kit Contents and Storage Conditions
Part | Part# | Description | Storage of Opened/Reconstituted |
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Human TGF-β1 Microplate | TBS3232A | 96-Well Polystyrene Microplate (12-strips of 8-wells) coated with a capture antibody specific for human TGF-946;1. | Return unused wells to the foil pouch containing the desiccant pack. Reseal along the entire edge of the zip seal. May be stored for up to 1 month at 2-8℃ |
Human TGF-β1 Standard | TBS3232B | 0.1mL of Recombinant human TGFβ1 protein (100 ng/mL). | Aliquot and store at -20℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
Detection A | TBS3232C | 2.2mL of human TGF-β1 antibody. | May be stored for up to 3 months at 2-8℃ |
Detection B | TBS3232D | 120μL of Streptavidin-HRP (100x). | |
Assay Diluent | TBS3232E | 12mL of buffered protein base with preservatives. | |
10x Wash Buffer | TBS3000W | 12mL of concentrated solution (10x). | |
TMB Substrate | TBS3000T | 12mL of ultra-sensitive TMB substrate. | |
Stop Solution | TBS3000S | 6mL of 2N sulfuric acid. |
Store the unopened kit at 2-8℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
Accurate Standard Curve
Additional information
SIZE (kit) | 1 Kit |
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Liu, X., Zhao, G., Huo, X., Wang, Y., Tigyi, G., Zhu, B. M., Yue, J., & Zhang, W. (2021). Adipose-Derived Stem Cells Facilitate Ovarian Tumor Growth and Metastasis by Promoting Epithelial to Mesenchymal Transition Through Activating the TGF-β Pathway. Frontiers in oncology, 11, 756011. https://doi.org/10.3389/fonc.2021.756011
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