TBS3232_TGFb1
TBS3232_ppt_Summary

Fast Human TGF-β1 ELISA (TBS3232)

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$450.00

SKU: TBS3232 Categories: ,
SKUStock SIZE (kit) PriceQuantity
TBS3232 Yes 1 x 96-Well Plate $450.00
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Description

The Fast Human TGF-β1 ELISA is a solid phase ELISA designed to measure human TGF-β1 levels in cell culture supernatants, serum, and plasma. The main feature of this the kit is its use of a novel fast approach to replace traditional methods. The kit is designed to ensure short steps, a short incubation time, and only one-step washing out. The measurement can be finished within 2 hours instead of the traditional 4-5 hours. The detection range is between 10-4,000 pg/mL. The levels of human TGF-β1 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human TGF-β1 protein.

 

Kit Contents and Storage Conditions

Part Name Part# Description Storage Conditions
Human TGF-β1 Microplate TBS3232A 96-well microplate (12-strips of 8-wells) coated with a Capture Antibody specific for human TGF-β1. The unused wells can be stored the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.
Human TGF-β1 Standard TBS3232B 40 µL of Recombinant human TGF-β1protein (100 ng/mL). Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A TBS3232C 200 µL of human TGF-β1antibody (12x).  

May be stored for up to

3 months at 2-8 °C.*
Detection B TBS3232D 250 µL of Streptavidin-HRP (60x).
Assay Diluent TBS3232E 25 mL of a buffered protein base with preservatives.
10x Wash Buffer TBS3000W 12 mL of concentrated solution (10x).
TMB Substrate TBS3000T 12 mL of ultra-sensitive TMB substrate.
Stop Solution TBS3000S 6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.

Human TGF-β1 Standard Preparation

 

Standard Curve


Research use only.

Additional information

SIZE (kit)

1 x 96-Well Plate

Datasheet:

TBS3232_human_TGFb1_elisa_Datasheet_V02_2026

MSDS:

TBS3232_Human_TBG-β1_ELISA_MSDS

Research use only.

Liu, X., Zhao, G., Huo, X., Wang, Y., Tigyi, G., Zhu, B. M., Yue, J., & Zhang, W. (2021). Adipose-Derived Stem Cells Facilitate Ovarian Tumor Growth and Metastasis by Promoting Epithelial to Mesenchymal Transition Through Activating the TGF-β Pathway. Frontiers in oncology, 11, 756011. https://doi.org/10.3389/fonc.2021.756011

Research use only.

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