dsRNA ELISA (TBS32119)
$780.00
| SKU | Stock | SIZE | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TBS32119 | Yes | 1 x 96-well plate | $780.00 |
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Description
The Tribioscience dsRNA Fast ELISA is designed to quantitatively detect dsRNA levels in different tissues, cells, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 3 hours, not needing 4-5 hours (Fig. 1). The detection range is from 20 to 1280 pg/mL. The levels of dsRNA samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural dsRNA protein. The dsRNA Fast ELISA can be used to determine dsRNA in multiple-species.
Typical Data
This standard curve (R2=0.9995) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MDD) of dsRNA is typically 10 pg/mL.
The Intra-assay CV is 3.79% the Inter-assay CV is ˂10%.
Specificity
This assay recognizes natural and recombinant dsRNA.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
| PART | PART# | DESCRIPTION | STORAGE OF OPENED/ RECONSTITUTED |
| dsRNA Microplate | TBS32119A | 96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for dsRNA. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| dsRNA Standard | TBS32119B | 30 µL of dsRNA control (64 ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS32119C | 220 µL of Biotin-dsRNA antibody (10x). |
May be stored for up to 3 months at 2-8 °C.* |
| Detection B | TBS32119D | 300 µL of Streptavidin-HRP (50x). | |
| Assay Diluent | TBS32119E | 15 mL of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 15 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
Alternative Names: Double-stranded RNA
Research use only.
Additional information
| SIZE | 1 x 96-well plate |
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