Cyclic AMP Colorimetric Assay (TBS2073)

Description

This assay kit is a competition enzyme-linked immunoassay designed to measure cAMP levels in cells or tissues. An anti-mouse IgG was pre-coated onto a microplate. The cAMP in the test sample competes with cAMP-HRP for binding to an anti-cAMP mouse monoclonal antibody. Following the incubation and wash step, an ultra-sensitive substrate TMB is added for color development. Because of the competitive nature of this assay, the color intensity is inversely proportional to the quantity of sample cAMP levels. Measurement of absorbance using the cAMP Standard allows calculating the absolute amount of cAMP in a sample. This assay provides a flexible, accurate, simple, and time-saving approach for cAMP detection.

 

Typical Data

This standard curve is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 2 is an example of typical Data.

Sensitivity

The minimum detectable dose (MOD) of cAMP is typically 1 nM.

The Intra-assay CV and the Inter-assay CV are ˂10%.

Specificity

This assay recognizes cAMP. No cross-reactivity with others.

 

Standard Preparation

 

Standard Curve (Fig.2)

 

Kit Content and Storage Conditions

Part	Part#	Description	Storage of Opened/Reconstituted
Precoated Microplate	TBS2073A	96 well polystyrene microplate (12 strips of 8 wells) coated with anti-mouse IgG antibody.	Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
cAMP Standard	TBS2073B	50 µL of cAMP (1 mM).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS2073C	110 µL of cAMP-HRP (25x).	May be stored for up to 3 months at 2-8 °C.
Detection B	TBS2073D	1.1 mL of anti-cAMP antibody (5x).
Assay Diluent	TBS2073E	20 mL of a buffered protein base with preservatives.
Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.

Additional information

SIZE (test)	100 Tests

Datasheet:

TBS2073 cAMP Colorimetric Assay_datasheet_v02_2024

MSDS:

TBS2073-cAMP Colorimetric Assay_MSDS_2024