BrdU Cell Proliferation Colorimetric Assay (TBS2086)

Description

BrdU Cell Proliferation Colorimetric Assay Kit is designed as a precise, fast and simple colorimetric method to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in proliferating cells.

5-bromo-2-deoxyuridine (BrdU) is a pyrimidine analog. It gets incorporated into the newly synthesized DNA of proliferating cells in place of thymidine. Tribioscience’s BrdU Cell Proliferation Colorimetric Assay Kit detects incorporated BrdU using an anti-BrdU antibody conjugate with Peroxidase (POD) and its substrate. The BrdU antibody POD conjugate allows direct detection of incorporated BrdU without the need for an additional secondary detection antibody. The extent of color development is proportional to the quantity of BrdU incorporated into the cells and can be used directly as an indicator of cell proliferation. Compared to other cell proliferation assays, this kit detects only the proliferating cells and not the seeded cells. This kit can detect as few as 50-100 proliferating cells.

Applications

• Detection and quantification of cell proliferation induced by growth factors, cytokines, mitogens, and nutrients.
• Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals.
• Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation.

Kit Components and Storage

Research use only.

Additional information

Datasheet:

TBS2086_BrdU cell proliferation Assay_v03_colorimetric_2025

MSDS:

TBS2086-BrdU_cell_prolif_colorimetric_MSDS_2024

Research use only.