Human EGF ELISA (TBS3249)

The Tribo^TM Fast Human EGF ELISA is designed to quantitatively detect Human EGF levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 3 hours, not needing 4-5 hours (Fig. 1). The detection range is from 3 to 250 pg/mL. The levels of human EGF samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human EGF protein.

 

Typical Data

This standard curve(R²=0.9995) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Sensitivity

The minimum detectable dose (MOD) of human EGF is typically 10 pg/ml.

The Intra-assay CV is 3.79% the Inter-assay CV is ˂10%.

Specificity

This assay recognizes natural and recombinant human EGF.

 

Simple Procedures

Simple Standard Preparation

Accurate Standard Curve

Part	Part#	Description	Storage of Opened/Reconstituted
Human EGF Microplate	TBS3249A	96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human EGF.	Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human EGF Standard	TBS3249B	30 µl of Recombinant human EGF protein (25ng/mL).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS3249C	2.1 ml of Biotin-Human EGF antibody.	May be stored for up to 3 months at 2-8 °C.
Detection B	TBS3249D	300 µl of Streptavidin-HRP.
Assay Diluent	TBS3249E	25 mL of a buffered protein base with preservatives.
Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.