Fast Mouse Phospho-Tau Thr181 ELISA (TBS3006)

$560.00

SKU: TBS3006 Category:
SKUStock SIZE PriceQuantity
TBS3006 Yes 1 x 96-well plate $560.00
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Description

Tribio Fast Mouse Phospho Tau Thr181 (p-Tau181) ELISA is designed for quantitative detection of mouse p-Tau181 in serum, plasma, and other biological samples. It uses a proprietary one-step detection method that simplifies the traditional protocol, making the assay fast, easy, and accurate. The total hands-on time is within 2 hours instead of 4–5 hours (Fig. 1). The detection range is 0.3–243 ng/mL, and mouse p-Tau181 levels show good linearity with the standard curve, enabling reliable quantification.

 

Typical Data

This standard curve(R2=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Sensitivity

The minimum detectable dose (MDD) of mouse ptau-181 is typically 300 pg/ml.

The Intra-assay CV and the Inter-assay CV are ˂10%.

Specificity

This assay recognizes natural and recombinant mouse pTau-181.

No cross-reactivity with others.

Simple Procedures

Simple Standard Preparation

TBS3006

Accurate Standard Curve

Kit Content and Storage Conditions

PART PART# DESCRIPTION STORAGE OF OPENED/ RECONSTITUTED
Mouse pTau-181 Microplate TBS3006A 96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for mouse p-Tau181. Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Mouse pTau-181 Standard TBS3006B 80 µL of Recombinant mouse p-Tau181 standard (2.43 μg/mL). Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.
Detection A TBS3006C 25 µL of p-Tau-181 mAb-HRP Conjugate (100x).
Assay Diluent TBS3006D 15 mL of a buffered protein base with preservatives. May be store for up to 3 months at 2-8 °C
Wash Buffer TBS3000W 12 mL of concentrated solution (10x).
TMB Substrate TBS3000T 12 mL of ultra-sensitive TMB substrate.
Stop Solution TBS3000S 6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.

 

Research use only.

Additional information

SIZE

1 x 96-well plate

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