Human BDNF ELISA (TBS32132)

$560.00

SKU: TBS32132 Category:
SKUStock SIZE PriceQuantity
TBS32132 Yes 96-well plate $560.00
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Description

Tribioscience’s Human BDNF ELISA is designed to quantitatively detect human BDNF levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast (Fig. 1). The detection range is from 12 to 9000 pg/mL. The levels of human BDNF samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human BDNF protein.

 

Typical Data

This standard curve (R2=0.998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Sensitivity

The minimum detectable dose (MDD) of human BDNF is typically 4 pg/mL.

The Intra-assay CV and the Inter-assay CV are ˂10%.

Specificity

This assay recognizes natural and recombinant human BDNF.

No cross-reactivity with others.

 

Simple Procedures (Fig. 1)

 

Simple Standard Preparation 

Standard Curve

Kit Content and Storage Conditions

PART PART# DESCRIPTION STORAGE OF OPENED/ RECONSTITUTED
Human BDNF Microplate TBS32132A 96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human BDNF. Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human BDNF Standard TBS32132B 8 µl of Recombinant human BDNF (1 µg/mL). Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.
Detection A TBS32132C 6 µl of biotin-human BDNF antibody. (500x)  

 

 

May be stored for up to 3 months at 2-8 °C.

Detection B TBS32132D 280 µL of streptavidin HRP (40x).
Assay Diluent TBS32132E 25 mL of a buffered protein base with preservatives.
Wash Buffer TBS3000W 12 mL of concentrated solution (10x)
TMB Substrate TBS3000T 12 mL of ultra-sensitive TMB substrate.
Stop Solution TBS3000S 6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration date.

The kit contains sufficient materials to run an ELISA on one 96 well plate.

Research use only.

Additional information

SIZE

96-well plate

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