MMLV Reverse Transcriptase Thermal stable (TBP0153)

Description

MMLV Reverse Transcriptase is an enzyme encoded by the Moloney Murine Leukemia Virus. As an RNA-dependent DNA polymerase, it synthesizes the first strand of complementary DNA (cDNA) from a single-stranded RNA template after a primer has been attached. MMLV RT is widely used in molecular biology to generate cDNA for applications such as RT-PCR and first-strand cDNA synthesis.

Features

• Low RNase H Activity: Exhibits weak RNase H activity, resulting in high cDNA yield and enabling the synthesis of more full-length cDNA.
• Thermally Stable: Optimal reaction temperature is 50°C, with a maximum of 55°C. Effectively overcomes RNA secondary structures, ensuring smooth and efficient reverse transcription.
• Broad Temperature Range: Functions efficiently between 37°C and 55°C, maintaining over 80% of peak activity between 42°C and 55°C.
• Enhanced Amplification Efficiency: Engineered mutations improve the enzyme’s binding affinity to RNA, significantly increasing amplification speed. Produces high-quality cDNA, ideal for cDNA library construction.

Product Details

Storage Buffer: 20 mMTris-HCl (pH7.5), 200 mM NaCl, 0.25 mM EDTA, 0.01% NP-40(v/v), 2.5 mM DTT, 50% glycerol (v/v).

5×Reaction Buffer: [5×RTBuffer] 250mM Tris-HCl (pH 8.3), 15mM MgCl₂, 375 mM KCl, 50mM DTT.

Storage: -20°C

Components

• MMLV(RNase H-)(200U/uL)
• 5×RT Buffer (with DTT)

Research use only.

Additional information

Datasheet:

TBP0153-MMLV Reverse Transcriptase Thermal stable_datasheet

MSDS:

TBP0153_MMLV Reverse Transcriptase Thermal stable_MSDS

Research use only.