Chymotrypsin Activity Colorimetric Assay (TBS2106C)

Description

Chymotrypsin (EC3.4.21.1) is a kind of digestive enzyme in the component of pancreatic juice to breakdown of proteins and polypeptides. It cleaves peptide bonds at side of the N-terminal to the hydrophobic amino acid which contains an aromatic ring in the side chain, such as tyrosine, tryptophan and phenylalanine. It is used to manage and reduce swelling and inflammation and is related to some diseases, such as Alzheimer’s disease, diabetes, and cancer.

Tribioscience Chymotrypsin Activity Colorimetric Assay provides a simple and sensitive method for monitoring chymotrypsin activity in biological samples (tissue, cells, serum, urine, stool). This assay uses a synthetic p-nitroaniline derivative (R-pNA) as its substrate. Chymotrypsin can specifically cleave the substrate to release pNA, a chromophore which can be measured at absorbance (OD 405 nm) as shown in Fig. 1.

Applications

This kit is used for the determination of chymotrypsin activity in biological samples.

Key features

• Flexible: can be used for 96 wells and 384 wells plate
• Simple: Just one-step: add-incubate-read model
• Time saving: a 30-minute reaction at 37˚C

Storage Conditions

The kit is shipped on ice and should be stored at -20°C for long-term storage. Shelf life of 12 months after receipt.

Preparation

Standard Curve

Research use only.

Additional information

Datasheet:

TBS2106C_chymotrypsin_col_Assay_V_02_25-Final

MSDS:

TBS2106C-Chymotrypsin Activity Colorimetric Assay_MSDS_2024

Research use only.