beta-Nicotimamide Adenine Dinucleotide Phosphate, Coenzyme (TBP0096)
$478.00 – $1,912.00
SKU | Stock | SIZE (mg) | Price | Quantity | ||
---|---|---|---|---|---|---|
TBP0096-1G | Yes | 1 G | $478.00 | |||
TBP0096-5G | Yes | 5 G | $1,912.00 |
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Description
Product Details
Form: Crystalline powder
Molecular Weight: 823.4
Solubility: Distilled water or dilute buffer
Stability: Store at -20° C (-4° F)
Purity: 98+%
Applications
NADP is used in the determination of amylase, creatine kinase, glucose-6-phosphate dehydrogenase, isocitrate dehydrogenase, and glucose.
Reagents
- 0.1M Triethanolamine buffer/substrate, pH 7.6: 1.86 g TEA¿HCl, 210 mg glycerate-3-P, 125 mg MgSO4¿7H 2O and 50 mg EDTA with 80 ml distilled water. Adjust pH to 7.6 with 1M NaOH-Na 2 and adjust volume to 100 ml with distilled water.
- 0.1 M MgCl2: 2.03 g MgCl2¿6H 2O in 100 ml distilled water.
- 14 mM D,L-Isocitrate: 4 mg D,L-isocitrate-Na3 in 1 ml TEA buffer.
- Isocitrate dehydrogenase, from porcine heart (5 mg protein/ml): 4 U/mg
Procedure
- Dissolve 50 mg NADP in 50 ml distilled water in a volumetric flask.
- Set spectrophotometer (equipped with strip chart recorder and temperature control) at 340 nm and 25°C.
- Into a cuvette, pipette the following: sample 0.10 ml Mix and read the absorbance A1.
Buffer (1) 2.50 ml
MgCl 2 (2) 0.10 ml
D,L-isocitrate (3) 0.10 ml
- Add 0.02 ml of the ICDH. Mix and read absorbance A2.
- Add an additional 0.02 ml ICDH. Mix and read absorbance A 3 (absorbance due to enzyme).
Additional information
SIZE (mg) | 5 G, 1 G |
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Datasheet:
TBP0096-beta-Nicotimamide Adenine Dinucleotide Phosphate
MSDS:
TBP0096_beta-Nicotimamide Adenine Dinucleotide Phosphate Safety Data Sheet
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