Hexokinase Activity Fluorometric Assay (TBS2107F)

Description

Hexokinase (HK, 6-Phosphate Glucose Kinase, ATP: D-Hexose 6-Phosphotransferase, ATP-dependent Hexokinase) plays an important role in glucose metabolism. Hexokinase deficiency leads to diseases such as X-linked muscular dystrophy and rare autosomal recessive hemolytic anemia. On the other hand, increased hexokinase activity is detected in various human tumors and is associated with metastasis.

Tribioscience’s Hexokinase Activity Fluorometric Assay kit provides a flexible, accurate, sensitive, and time-saving approach for detecting HK activity in a wide variety of samples. In this assay, HK converts glucose into glucose-6-Phosphate(G6P), which in turn undergoes a series of reactions and induces the Fluorometric-Probe to generate intense fluorescence product at 585 nm.

Features

• Flexible: Suitable for 96-well or 384 well plate.
• Accuracy: Absorbance measurement is proportional to the HK concentration..
• Sensitive: Can detect as low as 0.5mU/mL.
• Time-saving: Just incubate and read out style.

Applications

• Hexokinase activity in different samples like cells and tissues.

Standard Preparation

Standard Curve

Research use only.

Additional information

Datasheet:

TBS2107F_HK Flourimetric-assay_V02-2024

MSDS:

TBS2107F-HK Fluorometric assay_MSDS_2024

Research use only.