ß-Galactosidase, Enzyme Activity (TBP0018)
$150.00 – $600.00
SKU | Stock | SIZE | Price | Quantity | ||
---|---|---|---|---|---|---|
TBP0018-1KU | Yes | 1000 U | $150.00 | |||
TBP0018-5KU | Yes | 5000 U | $600.00 |
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Description
Preparation and Specification
Appearance: White amorphous powder, lyophilized.
Activity: GradeⅡ 500U/mg-solid or more
Contaminants: α-galactosidase <1×10⁻⁴%
α-glucosidase <1×10⁻⁴%
ß-glucosidase <2×10⁻³%
α-mannosidase <1×10⁻⁴%
ß-mannosidase <1×10⁻⁴%
proteinase <10mAbs/mg-P
Stabilizer: Mg⁺⁺
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: 540,000
Isoelectric point: 4.6
Michaelis constants: 3.0×10⁻⁴M (o-Nitrophenyl-ß-D-galactoside), 6.7×10⁻⁵M (p-Nitrophenyl-ß-D-galactoside), 2.3×10⁻⁴M
(Phenyl-ß-D-galactoside), 2.5×10⁻³M (Lactose)
Structure: The enzyme is composed of four identical subunits having a molecular weight of ca.135,000. The amino acid analysis indicates approximately 1,170 residues per subunit.
Inhibitors: p-Chloromercuribenzoate, lodoacetamide, heavy metal ions (Zn⁺⁺, Fe⁺⁺⁺, Cd⁺⁺, Cu⁺⁺, Pb⁺⁺, Ag⁺, Hg⁺⁺), lonic detergents (SDS, DAC, etc.)
Optimum pH: 7.0-7.5
Optimum temperature: 50-55℃
pH Stability: pH 6.5-8.5 (25℃, 20hr)
Thermal stability: below 50℃ (pH 7.3, 15min)
Applications
This enzyme is useful for structural investigation of carbohydrates, the determination of lactose (foodstuff analysis) and as an enzyme label for enzyme immunoassay.
Additional information
SIZE | 1000 U, 5000 U |
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