Leucine dehydrogenase, Enzyme Activity (TBP0033)
$125.00 – $499.00
SKU | Stock | SIZE | Price | Quantity | ||
---|---|---|---|---|---|---|
TBP0033-100U | Yes | 100 U | $125.00 | |||
TBP0033-500U | Yes | 500 U | $499.00 |
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Description
Preparation and Specification
Appearance: White amorphous powder, lyophilized
Activity: GradeⅡ 20U/mg-solid or more (containing approx. 70% of stabilizers)
Contaminants: Leucylpeptide decomposing enzymes
(Leu-Val) ≤1.0×10⁻²%
(Leu-Gly-Gly) ≤1.0×10⁻²%
NADH oxidase ≤1.0×10⁻²%
Stabilizers: 2-Mercaptoethanol, L-cysteine, dithiothreitol, ethylenediaminetetraacetate
Properties
Stability: Stable at -20°C for at least One year
Molecular weight: 245,000
Michaelis constants: 1.0×10⁻³M (L-Leucine), 3.9×10⁻⁴M (NAD⁺), 3.5×10⁻⁵M (NADH),
3.1×10⁻⁴M [α-Ketoisocaproate (α-KIC)], 2.0×10⁻¹M (NH₃)
Structure: 6 subunits per enzyme molecule
Inhibitors: Na₂S, Hg⁺⁺, Cu⁺⁺, Co⁺⁺, Mg⁺⁺, p-chloromercuribenzoate
Optimum pH: 10.5-10.8 (L-Leu→α-KIC), 9.4 (α-KIC→L-Leu)
Optimum temperature: above 70°C
pH Stability: pH 5.5-10.5 (25°C, 20hr)
Thermal stability: below 60°C (pH 6.9, 10min)
Applications
This enzyme is useful for enzyme determination of L-leucine and the activity of leucine amino-peptidase.
Additional information
SIZE | 100 U, 500 U |
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