Column-Pure Fungi RNA Isolation Kit (TBS6100)

$162.00

SKU: TBS6100 Category:
SKUStock SIZE PriceQuantity
TBS6100 Yes 50 Preparations $162.00
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Description

Column-Pure Fungi RNA Isolation Kit is designed for preparation of high quality total RNA from a wide range of fungal species. Fungal samples are lysed and homogenized by Buffer Rlysis-FG. RNA in the whole homogeneity is selectively absorbed on the spin column and other impurities are washed away. Total RNA is eluted from the membrane in the presence of RNase-Free Water in the final step.

 

3-5 μg total RNA can be purified from 30 mg filamentous fungi using this kit. Purified RNA is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly A+ purification, nuclease protection and in vitro translation.

 

Component

Product Component Quantity (50 Preps)
Buffer Rlysis-FG 25 ml
Universal GT Solution* 18 ml
Universal NT Solution* 6 ml
RNase-free Water 5 ml
Column-Pure Spin Column (with 2.0-ml Collection Tube) 50

*Universal GT Solution and Universal NT Solution are supplied in a concentrated form. Before use, add 12 ml 96-100% ethanol to 18 ml concentrated Universal GT Solution and 24 ml 96-100% ethanol to 6 ml concentrated Universal NT Solution to make the complete solutions.

 

Store at 4°C

 

Features

  • Fast. Using a rapid spin-column format, the entire procedure takes approx 20 minutes.
  • High quality of RNA. OD260/280 of purified RNA is generally >1.8.
  • Intact RNA: NO RNA degradation and integrity maintained.
  • Economical. Simple, cost effective method of RNA isolation.

Research use only.

Additional information

SIZE

50 Preparations

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