Column-Pure Animal Genomic DNA Kit (TBS6102)

Description

Column-Pure Animal Genomic DNA Isolation Kit is a quick and easy spin column method for the isolation of genomic DNA from various animal tissues or cells. No phenol, chloroform, or other hazardous organic solutions are required. Proteins are degraded by Proteinase K, DNA is bound to the silica-based filter in the spin column, impurities are washed away, and the purified gDNA is eluted from the binding matrix of the column. The isolated genomic DNA is of excellent quality for PCR, restriction digestion, and other downstream applications. The kit contains all necessary tubes for the complete experiment in isolation of genomic DNA.

Component

1. ACL Solution may form a precipitate upon storage. If necessary, dissolve the precipitate by warming the solution to 37ºC.
2. Before use, add 1 ml of sterilized water to the tube containing 20 mg of Proteinase K. Keep solution at -20ºC.
3. Before use, add 48 ml of 100% ethanol to 12 ml Wash Solution.
4. Elution Buffer is 2.0 mM Tris-HCl pH 8.0~8.5, although TE buffer pH 8.0 or water can be used, yield is generally 10% lower.

Store at 4°C. Store Proteinase K at -20°C

Research use only.

Additional information

Datasheet:

TBS6102-Column-Pure Animal Genomic DNA Isolation Kit_datasheet

MSDS:

TBS6102_Column-Pure Animal Genomic DNA Isolation Kit_MSDS

Research use only.