Tribo™ Human AFP ELISA Kit (TBS3212)

Description

Tribioscience’s Fast Human AFP ELISA is designed to quantitatively detect human AFP levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The hands-on time can be within 2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 10 to 100000 pg/mL. The levels of human AFP samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human AFP protein.

 

Typical Data

This standard curve (R²=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Sensitivity

The minimum detectable dose (MOD) of human is typically 200 pg/ml. The Intra-assay CV and the Inter-assay CV are ˂10%.

Specificity

This assay recognizes natural and recombinant human pTau-217. No cross-reactivity with others.

 

Simple Procedures (Fig. 1)

 

Simple Standard Preparation

 

Accurate Standard Curve

 

 

Kit Content and Storage Conditions

Part	Part#	Description	Storage of Opened/Reconstituted
Human AFP Microplate	TBS3212A	96 well strip microplate (12 strips of 8 wells) coated with a human AFP monoclonal antibody.	Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human AFP Standard	TBS3212B	50 µL of Recombinant human AFP protein (ng/mL).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS3212C	2.2 mL of HRP-Human AFP antibody.	May be stored for up to 3 months at 2-8 °C.
Assay Diluent	TBS3212D	15 ml of a buffered protein base with preservatives.
Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.

Additional information

SIZE	1 x 96-well plate

Datasheet:

TBS3212_human-AFP-elisa_datasheet-V02-2024

MSDS:

TBS3212_human-AFP_ELISA_MSDS