Tribo™ Human β2-microglobulin ELISA Kit (TBS3218)

$430.00

SKU: TBS3218 Category:
SKUStock SIZE PriceQuantity
TBS3218 Yes 1 x 96-well plate $430.00
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Description

The TribioTM Fast Human β2M ELISA is designed to quantitatively detect Human β2M levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 3 hours, not needing 4-5 hours (Fig. 1). The detection range is from 15 to 1000 pg/mL. The levels of human β2M samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human β2M protein.

 

Typical Data

This standard curve(R2=0.9995) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Sensitivity

The minimum detectable dose (MOD) of human β2M is typically 10 pg/ml.

The Intra-assay CV is 3.79% the Inter-assay CV is ˂10%.

Specificity

This assay recognizes natural and recombinant human β2M.

 

Simple Procedures

TBS3235-0

Simple Standard Preparation

Accurate Standard Curve

 

Kit Content and Storage Conditions

Part Part# Description Storage of Opened/Reconstituted
Human β2M Microplate TBS3218A 96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human β2M. Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human β2M Standard TBS3218B 30 µl of Recombinant human β2M protein (50ng/mL). Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A TBS3218C 2.1 ml of Biotin-Human β2M antibody. May be stored for up to 3 months at 2-8 °C.
Detection B TBS3218D 300 µl of Streptavidin-HRP.
Assay Diluent TBS3218E 25 mL of a buffered protein base with preservatives.
Wash Buffer TBS3000W 12 mL of concentrated solution (10x).
TMB Substrate TBS3000T 12 mL of ultra-sensitive TMB substrate.
Stop Solution TBS3000S 6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.

Additional information

SIZE

1 x 96-well plate

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