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Tribioscience’s Fast Human D-Dimer ELISA is designed to quantitatively detect human D-Dimer levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 1 hour, not need 4-5 hours (Fig. 1). The detection arrangement is from 2 to 1458ng/mL. The levels of human D-Dimer samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for human D-Dimer.
The minimum detectable dose (MOD) of human is typically 1ng/ml.
The Intra-assay CV is 4.79% the Inter-assay CV is ˂10%.
Diagram for Human D-Dimer Standard Preparation
Kit Content and Storage Conditions
|Part||Part#||Description||Storage of Opened/Reconstituted|
|Human D-Dimer Microplate||TBS3206A||96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human D-Dimer.||Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.|
|Human D-Dimer Standard||TBS3206B||100 µl of Recombinant human D-Dimer (14.58 µg/mL).||Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.|
|Detection A||TBS3206C||2.1 ml of HRP- human D-Dimer antibody.||May be stored for up to 3 months at 2-8 °C.|
|Assay Diluent||TBS3206D||12 ml of a buffered protein base with preservatives.|
|Wash Buffer||TBS3000W||12 mL of concentrated solution (10x).|
|TMB Substrate||TBS3000T||12 mL of ultra-sensitive TMB substrate.|
|Stop Solution||TBS3000S||6 mL of 2 N sulfuric acid.|
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
1 X 96-Well Plate