Human D-Dimer ELISA; 1 step and 1hr.

Tribioscience’s Fast Human D-Dimer ELISA is designed to quantitatively detect human D-Dimer levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not need 4-5 hours (Fig. 1). The detection range is from 2 to 1458 ng/mL. The levels of human D-Dimer samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for human D-Dimer.

Sensitivity

The minimum detectable dose (MOD) of human is typically 1 ng/ml.
The Intra-assay CV is 4.79% the Inter-assay CV is ˂10%.

 

Simple Procedures (Fig.1)

Simple Standard Preparation

Accurate Standard Curve

Kit Content and Storage Conditions

Part	Part#	Description	Storage of Opened/Reconstituted
Human D-Dimer Microplate	TBS3206A	96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human D-Dimer.	Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human D-Dimer Standard	TBS3206B	100 µl of Recombinant human D-Dimer (14.58 µg/mL).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS3206C	2.1 ml of HRP- human D-Dimer antibody.	May be stored for up to 3 months at 2-8 °C.
Assay Diluent	TBS3206D	12 ml of a buffered protein base with preservatives.
Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.

 

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