Human 7-DHC ELISA (TBS32128)

$460.00$790.00

Synonym: 7-Dehydrocholesterol

SKU: TBS32128 Category:
SKUStock SIZE PriceQuantity
TBS32128-48 Yes 1 x 48-well plate $460.00
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TBS32128-96 Yes 1 x 96-well plate $790.00
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Description

7-Dehydrocholesterol (7-DHC) is a cholesterol precursor in the serum and is photochemically converted to vitamin D3 in the skin. Its functioning serves as a provitamin-D3 to vitamin D in the body upon exposure to ultraviolet B (UVB) radiation from sunlight. A deficiency in 7-DHC can lead to various health issues, such as increased risk of osteoporosis, cancer and higher susceptibility to infections. The Tribo 7-DHC ELISA Kit can quickly, sensitively, and accurately determine the presence of 7-DHC in CSF, urine, serum and other biological samples.

Intended Use

The Tribo 7-DHC ELISA Kit utilizes competitive ELISA for the quantitative and qualitative analysis of 7-DHC in biological samples. The limit of detection (LOD) of 7-DHC in ELISA Kit is 0.2ng/ml.

Typical Data

This standard curve is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Simple Procedures (Fig. 1)

Simple Standard Preparation

Accurate Standard Curve

Kit Content and Storage Conditions

PART PART# 48 Strip-well ELISA 96 Strip-well ELISA DESCRIPTION Storage
Pre-coated Microplate TBS32128A 48 Strip-well plate 96 Strip-well plate  48 or 96 Strip-well microplate coated with an antibody specific to 7-DHC. 4℃
7-DHC Standard TBS32128B 300 µL 600 µL  Total 7-DHC (200 ng/mL). -20℃
Detection A (100x) TBS32128C 11 µL 22 µL  Biotin-labeled Competitor (100x). -20℃
Detection B (100x) TBS32128D 55 µL 110 µL  Streptavidin-HRP (100x). -20℃
Assay Diluent TBS32128E 8 mL 13 mL  Assy Diluent. 4℃
Wash Buffer (10x) TBS3000W 12 mL 12 mL  Concentrated Buffer (10x). 4℃
TMB Substrate TBS3000T 6 mL 12 mL  Ultra-sensitive TMB substrate. 4℃
Stop Solution TBS3000S 3 mL 6 mL  2 N sulfuric acid. 4℃

The kit contains sufficient materials to run an ELISA on a half or one 96 well plate.

Research use only.

Additional information

SIZE

1 x 48-well plate, 1 x 96-well plate

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