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Interferon-gamma (IFN-γ, also known as type II interferon) is an important immunoregulatory cytokine through its anti-viral activity. IFN-γ is produced by a number of cell types under inflammatory conditions, including dendritic epidermal cells, T cells, keratinocytes, peripheral blood T cells, mast cells, neurons, CD8+T cells, macrophages, B cells, neutrophils, NK cells, CD4+T cells, and testicular spermatids. It plays key roles in host defense and in the progression of inflammatory diseases such as autoimmunity and atherosclerosis by exerting anti-viral, anti-proliferative, and immunoregulatory activities.
The Tribo™ Fast Monkey IFN-γ ELISA kit is a solid phase ELISA designed to measure monkey IFN- γ levels in cell culture supernatants, serum, and plasma. The main feature of this kit is its use of a novel fast approach to replace the traditional methods. The measurement can be finished within 1.5 hours instead of the traditional 4-5 hours. The detection range is between 20 and 15,000 pg/mL. The levels of monkey IFN- γ samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural monkey IFN-γ protein.
Principle of the Assay:
This assay employs the quantitatve e sandwich enzyme immunoassay technique. A monoclonal antibody specific to monkey IFN-γ was pre-coated onto a microplate. Standards and samples can then be pipetted into the wells and incubated with an HRP-conjugated detection antibody specific for monkey IFN-γ. Following a wash to remove any unbound antibody and samples, an ultra-sensitive TMB substrate solution can be added to the wells to develop color. The color intensity is proportional to the amount of IFN-γ bound in the initial step. The intensity of the color is measured by plate reader at 450nm.
Kit Contents & Storage Conditions:
Assay DiluentTBS3231D12mL of buffered protein base with perservatives.
|Part||Part#||Description||Storage of Opened/Reconstituted|
|Monkey IFN-γ Microplate||TBS3231A||96-Well Polystyrene Microplate (12-strips of 8-wells) coated with a polyclonal antibody specific for monkey IFN-γ||Return unused wells to the foil pouch containing the desiccant pack. Reseal along entire edge of the zip seal. May be stored for up to 1 month at 2-8℃|
|Monkey IFN-γ Standard||TBS3231B||0.1mL of Recombinant monkey IFN-γ protein (145.8 ng/mL).||Aliquot and store at -20℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.|
|Detection A||TBS3231C||2.2mL of HRP-Monkey IFN-γ antibody.||May be stored for up to 3 months at 2-8℃|
|Wash Buffer Concentrate||TBS3000W||12mL of concentrated solution (10x).|
|TMB Substrate||TBS3000T||12mL of ultra-sensitive TMB substrate.|
|Stop Solution||TBS3000S||6mL of 2N sulfuric acid.|
|Plate Sealers||N/A||Adhesive Strips|
Store the unopened kit at 2-8℃. Do not use past kit expiration date.
The kit contains sufficient materials to run an ELISA on one 96-well plate