CD26 (DPP4) Activity Colorimetric Assay (TBS2210)

Description

CD 26 (dipeptidyl peptidase-4/DPP4) is a transmembrane glycoprotein expressed on many cell types, including immune cells, epithelial cells, and endothelial cells. Its enzymatic activity: cleaves dipeptides from the N-terminus of polypeptides when the second amino acid is proline or alanine. It is used to manage and reduce inflammation and is related to some diseases, such as Alzheimer’s disease, diabetes, and cancer.

Tribioscience CD26 Activity Colorimetric Assay provides a simple and sensitive method for monitoring CD26 activity in biological samples (tissue, cells, serum, urine, stool). This assay uses a synthetic p-nitroaniline derivative (R-pNA) as its substrate. CD26 can specifically cleave the substrate to release pNA, a chromophore which can be measured at absorbance (OD 405 nm) as shown in Fig. 1.

Fig. 1 

Features

• Flexible: can be used for 96 wells and 384 wells plate
• Simple: Just one-step: add-incubate-read model
• Time saving: a 30-minute reaction at 37˚C

Applications

• This kit is used for the determination of CD26 activity in biological samples.

Kit Contents

TYPICAL DATA

This standard curve is provided for demonstration only as below Fig.2. A standard curve should be generated for each set of samples assayed.

Typical data of different concentrations of enzyme with substrate shown as Fig. 3.

Research use only.

Additional information

Datasheet:

TBS2210-CD26_col_Assay_V_02_26

Research use only.