Beta-N-Acetylglucosaminidase (Beta-NAG) Activity Colorimetric Assay (TBS2105)

Description

β-Hexosaminidase (β-N-Acetylglucosaminidase, NAGase, EC 3.2.1.52) is a lysosomal enzyme that is expressed in various tissues, including kidneys, liver, lungs, and mast cells. Elevated enzyme levels are associated with many disorders, such as Tay-Sachs and Sandhoff disease, inflammation, and abnormal immune responses.

The Beta-Hexosaminidase Activity Colorimetric Assay provides a simple and sensitive method for monitoring hexosaminidase activity in biological samples (tissue, cells, serum, urine). This assay uses a synthetic p-nitrophenol derivative (R-pNP) as its substrate and releases pNP which can be measured at absorbance (OD 405 nm). The assay can detect as low as 50 µU of NAGase activity in a variety of samples.

Applications

This kit is used for the determination of hexosaminidase activity in biological samples.

Key features

• Fast and sensitive: Linear detection range (20 µL sample): 0.05 to 50 U/L for a 30-minute reaction at 37˚C.
• High throughput: This can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Storage Conditions

This kit is shipped on ice. Store all components at -20℃ in a non-frost-free freezer. Shelf life of 12 months after receiving.

Preparation

Standard Curve

Research use only.

Additional information

Datasheet:

TBS2105_beta-NAG_V_06_24-update

MSDS:

TBS2105-Beta-NAG Colorimetric Assay_MSDS_2024

Research use only.

Sun, Y., Wu, Y., He, Z., Wang, Y., Hou, W., Cao, Y., Zhou, Q., & Zhang, R. (2025). Type III CRISPR-mediated flexible RNA excision with engineered guide RNAs. Molecular cell, 85(5), 989–998.e4. https://doi.org/10.1016/j.molcel.2025.01.021

Research use only.