α-Glucosidase Activity Assay (TBS2208)

Description

ɑ-Glucosidase (α-GC), also known as α-D-glucoside hydrolase is a crucial enzyme for carbohydrate digestion. It can convert complex sugars into glucose with significant roles in metabolism, diabetes treatment, and drug discovery.

Fig. 1: Assay Principle

Tribioscience ɑ-Glucosidase Activity Colorimetric Assay provides a simple and sensitive method for monitoring ɑ-glucosidase activity in biological samples (tissue, cells, serum, urine, stool). This assay uses a synthetic p- ɑ-Glucosidase substrate (p-NPG) to release p-nitrophenol (pNP), which turns yellow under alkaline conditions. The absorbance of pNP is measured at 405 nm as shown in Fig. 1.

APPLICATIONS

Determination of ɑ-Glucosidase activity in biological samples.

KEY FEATURES

Flexible: can be used for 96 wells and 384 wells plate.

Simple: Just one-step: add-incubate-read model.

Time saving: a 30-minute reaction at 37˚C

KIT CONTENTS

STORAGE CONDITIONS

The kit is shipped on ice and should be stored at -20°C for shelf life of 12 months after receipt.

TYPICAL DATA

This standard curve is provided for demonstration only as Fig.3. A standard curve should be generated for each set of samples assayed.

TYPICAL DATA: Different concentrations of enzyme with substrate shown as Fig. 4.

Research use only.

Additional information

Datasheet:

TBS2208 a-GlucosidaseActivity_col_Assay_Datasheet_V01

MSDS:

TBS2208-α-Glucosidase Activity Assay_MSDS

Research use only.