Canine IL-8/CXCL8 Fast ELISA (TBS33008)

Description

Interleukin 8 (IL-8, also as CXCL8) is a chemokine with a defining CXC amino acid motif, and has tumorigenic and proangiogenic properties. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli (IL1, TNF, LPS, etc). This chemokine has crucial roles in various pathological conditions such as chronic inflammation and cancer. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer.

The Canine (Dog) IL-8 Fast ELISA is a solid phase ELISA designed to measure canine/dog IL-8 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1-2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 8 to 2000 pg/mL. The levels of canine IL-8 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural canine IL-8 protein.

Typical Data

This standard curve (Fig 3. R²=0.9992) is provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Sensitivity

The minimum detectable dose (MOD) of canine IL-8 is typically 7.8 pg/ml.
The Intra-assay CV is 3.8%
The inter-assay CV is ˂10%.

Specificity

This assay recognizes natural and recombinant canine IL-8.

 

Simple Procedures (Fig. 1)

 

Standard Preparation 

 

Accurate Standard Curve

 

Kit Content and Storage Conditions

Part	Part#	Description	Storage of Opened/Reconstituted
Canine IL-8 Microplate	TBS33008A	96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for canine IL-8.	The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.
Canine IL-8 Standard	TBS33008B	15 µL of Recombinant canineIL-8 protein (100 ng/mL).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS33008C	2.2 ml of canine IL-8 antibody.	May be stored for up to 3 months at 2-8 °C.
Detection B	TBS33008D	120 µL of Streptavidin-HRP (100x)
Assay Diluent	TBS33008E	25 ml of a buffered protein base with preservatives.
10x Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.

Additional information

SIZE	96-well plate

Datasheet:

TBS33008_canine_IL8_elisa_08_V022024

MSDS:

TBS33008_canine_IL8_ELISA_MSDS