Adenosine 5′-diphosphate, Coenzyme (TBP0092)
$81.00 – $324.00
SKU | Stock | SIZE (mg) | Price | Quantity | ||
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TBP0092-1G | Yes | 1 G | $81.00 | |||
TBP0092-5G | Yes | 5 G | $324.00 |
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Description
Product Details
Form: Crystalline powder
Molecular Weight: 507.20
Solubility: Distilled water or dilute buffer
Stability: Store at -20° C (-4° F)
Assay Method
The assay is based on the reactions described by J.L. Strominger, L.A. Heppel, E.S. Maxwell, Biochim.
Applications
Adenosine 5′-diphosphate (ADP) is used in the determination of creatine kinase, glutamate dehydrogenase, urea and ammonia.
Reagents
- Triethanolamine buffer, 0.1M, pH 7.6: 1.86 g TEA• HCl in 80 ml distilled water. Adjust to pH 7.6 with 1.0 M NaOH, adjust volume to 100 ml with distilled water.
- 14mM NADH:10 mg NADH-Na2 with 1 ml distilled water.
- 0.5 M MgSO4/2 M KCl: 1.23 g MgSO4• 7H2O and 1.49 g KCl in 10 ml distilled water.
- 32 mM PEP: 5 mg PEP-(CHA)3 in 1 ml solution (3).
- LDH, from rabbit muscle: 5 mg protein/ml (~550 U/mg).
- PK, from rabbit muscle: 10 mg protein/ml (200 U/mg).
Procedure
- Dissolve 50 mg ADP in 50 ml distilled water in a volumetric flask.
- Set spectrophotometer (equipped with strip chart recorder and temperature control) at 340 nm and 25° C.
- Into a cuvette, pipette the following:
Buffer (1) 2.70 ml
NADH (2) 0.05 ml
PEP (4) 0.15 ml
LDH (5) 0.02 ml
sample 0.10 ml
- Mix and read the absorbance A
- Start the reaction by adding 0.02 ml PK (6) and read the absorbance A2.
- When the reaction is complete add an additional 0.02 ml PK (6). Mix and read the absorbance A3.
Additional information
SIZE (mg) | 5 G, 1 G |
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