Malate dehydrogenase, Enzyme Activity (TBP0035)

Description

Preparation and Specification

Appearance: Slightly yellowish amorphous powder, lyophilized

Activity: GradeⅡ　40U/mg-solid or more

Contaminants: Glutamate oxaloacetate transaminase ≤1.0×10⁻³%

Lactate dehydrogenase ≤1.0×10⁻³%

NADH oxidase≤1.0×10⁻³%

 

Properties

Stability: Stable at -20°C for at least One year

Molecular weight: approx. 140,000

Isoelectric point: pH 4.8±0.1

Michaelis constants: 5.4×10⁻⁵M (L-Malate), 5.0×10⁻⁶M (Oxaloacetate), 8.1×10⁻⁶M (NADH)

Structure: 4 subunits per enzyme molecule

Inhibitors: Hg⁺⁺

Optimum pH: 8.0

Optimum temperature: 70°C

pH Stability: pH 3.0-9.0 (25°C, 20hr)

Thermal stability: below 70°C (pH 7.5, 15min)

 

Applications

This enzyme is useful for enzymatic determination of L-malate and of glutamate oxaloacetate transaminase (GOT) in clinical analysis.

Additional information

SIZE (mg)	10 MG, 50 MG

Datasheet:

TBP0035-Malate dehydrogenase

MSDS:

TBP0035_Malate dehydrogenase Safety Data Sheet