D-Lactate dehydrogenase, Enzyme Activity (TBP0031)

Description

Preparation and Specification

Appearance: White amorphos powder, lyophilized

Activity: Grade II 900 U/mg-solid or more

Contaminants: NADH oxidase ≤1.0×10⁻³%

Malate dehydrogenase ≤1.0×10⁻²%

GOT ≤5.0×10⁻³%

GPT ≤5.0×10⁻³%

Myokinase ≤1.0×10⁻²%

Pyruvate kinase ≤1.0×10⁻³%

 

Properties

Stability: Stable at -20°C for at least One year

Molecular weight: approx. 140,000

Isoelectric point: 4.0

Michaelis constant: 6.4×10⁻⁴M (pyruvate, pH 7.0)

Inhibitors: Ag²+, Hg²+, SH-reagents

Optimum pH: 5.0-7.0

Optimum temperature: 30-37°C

pH Stability: pH 5.0-9.0 (25°C, 48hr)

Thermal stability: below 45°C (pH 7.0, 15min)

 

Applications

This enzyme is useful for enzymatic determination of numerous metabolites, e.g. ATP, ADP, glucose, creatinine, pyruvate, lactate and glycerol, and of enzyme activities, e.g. GPT, PK, and CPK when coupled with the related enzymes.

Additional information

SIZE	1000 U, 5000 U

Datasheet:

TBP0031-D-Lactate dehydrogenase

MSDS:

TBP0031_D-Lactate dehydrogenase Safety Data Sheet