Mycoplasma positive control
Mycoplasma positive control
A built-in Mycoplasma positive control is essential in PCR-based mycoplasma detection, ensuring assay performance and reliability of results. In every PCR test, both the Positive Control (5 µL DNA/reaction) and Negative Control (5 µL DNA/reaction) must be included to confirm that the amplification process is functioning correctly. The presence of a reliable positive control verifies that the assay conditions and reagents are performing as expected, preventing false-negative results and maintaining assay integrity.
The Mycoplasma positive control can also be used to generate a standard curve through 10× sequential dilutions, allowing for precise quantification of the assay’s detection capability. The copy number, clearly labeled on the vial, provides a reference for evaluating assay sensitivity and consistency across tests. This built-in feature supports accurate interpretation and comparability of data in routine quality control.
During data analysis, a sample is considered positive when the Ct value is ≤ 37 and all controls perform normally, and negative when the Ct value is ≥ 38 with normal control reactions. The PCR internal control must also be positive in all reactions to indicate successful amplification. If any of the controls, such as the positive, negative, or blank control, fail, the test must be repeated. This strict control system ensures dependable assay performance and trustworthy results in mycoplasma detection.
